Biometra TOne – PCR Thermal Cycler (2024)

Biometra TOne – PCR Thermal Cycler (1)
  • Reliable partner thanks to robust design and easy to use software
  • Excellent heating and cooling rates for specific results
  • Superior temperature uniformity for reproducible results across the entire sample block

Featuring top quality and detailed precision, Biometra thermal cyclers make your lab workday easier and ensure excellent results.

More than 30 Years PCR Competence for Research and Routine Use

In the Analytik Jena thermal cycler portfolio, every user will find the right device: The Biometra product family offers PCR devices ranging from standard to premium equipment – all designed in line with the common principles of being reliable, convenient, and precise.

  • Designed for error-free use in versatile laboratory environments: universal block design and heating lid with High-Performance Smart Lid (HPSL) technology for unrestricted use of different plastic containers and plates
  • Easily optimize your PCR protocols using the Linear Gradient Function (LGT)
  • Full reliability of your results: The high-precision block temperature control system provides high heating and cooling rates and excellent temperature hom*ogeneity for constant conditions across all sample positions.

Download Product Information

Brochures

Title Language Info
Broschüre Biometra Thermocycler-Serie (DE) de PDF, 856 KB
Brochure PCR Biometra Thermal Cycler Series (EN) en PDF, 908 KB
Product Guide Consumables for PCR/qPCR (EN) en PDF, 5 MB
Broschüre Biometra TMS Temperaturmesssystem (DE) de PDF, 1 MB
Brochure Biometra TMS Temperature Measuring System (EN) en PDF, 1 MB
Flyer Biometra TSuite Software (DE) de PDF, 2 MB
Flyer Biometra TSuite Software (EN) en PDF, 3 MB
Br NGS Library Preparation (EN) en PDF, 2 MB
Brochure Product Guide Life Science (EN) en PDF, 2 MB
Broschüre Produktführer Life Science (DE) de PDF, 2 MB

Tradition Meets Innovation

Analytik Jena can look back on a long tradition of developing high-quality, highly precise analytical systems. The Biometra thermal cyclers stand for high-performance sample blocks. Amplification results are always excellent and reliable with ideal reproducibility.

The Biometra thermalcycler family offers a range of high-quality models to meet individual user needs. All models can also be controlled and monitored live from the desk via the optional Biometra TSuite thermal cycler management software.

The Biometra TOne is a high-performance system with a 96-well block, also available with a gradient function. The combination of excellent technical data and an attractive price makes it the right choice for many research and routine laboratories. The robust and easy-to-control thermal cycler is well suited for the most common applications in 96 well formats with optional 12 column gradient function. Fast pinpoint results can be gained, thanks to a high heating rate of 4 °C/s.

Products

Biometra TOne 96 G, 230 V

High-quality thermal cycler with 96 well aluminum block and gradient function.

It is available as 100 V, 115 V and 230 V version.

Order number: 846-2-070-301

Biometra TOne 96, 230 V

High-quality thermal cycler with 96 well aluminum block.

It is available as 100 V, 115 V and 230 V version.

Order number: 846-2-070-311

Technical Data

Sample Block

Block capacity

Proposed sample volume

Maximum Heating

Average Heating

Maximum Cooling

Dimension (width x depth x height)

Average Cooling

Temperature uniformity

Gradient

Max./Min. gradient

Adjustable gradient range

Block exchange

Tempering method

Standby temperature

Temperature control mode

Adjustable temperature range

Temperature control accuracy

Lid type

Space requirement (width x depth x height)

Lid temperature

Compatible sealing systems

Consumable compatibility

Time increment

Temperature increment/decrement

Biometra TOne 96 G, 230 V
846-2-070-301

Aluminum, special alloy 96 x 0.2 mL tubes, 96 well microplates or 8 well strips 5 - 50µL 4.0°C/s 3.7°C/s 3.3°C/s 260 mm x 430 mm x 241 mm 3.0°C/s +/- 0,20 °C at 55 °C after 15 s Yes (semi-linear) 20 °C/0.1°C 12 rows 20 - 99°C No Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0.1°C High-Precision Smart Lid (HPSL) 260 mm x 440 mm x 241 mm 30 - 110°C Sealing foils, sealing mats, lid stripes, tube lids PCR tubes, high- and low-profile microplates (non-skirted, semi-skirted, full-skirted) 1 - 240s/cycle +/- 0.1 - 20 °C

Biometra TOne 96, 230 V
846-2-070-311

Aluminum, special alloy 96 x 0.2 mL tubes, 96 well microplates or 8 well strips 5 - 50µL 4.0°C/s 3.7°C/s 3.3°C/s 260 mm x 430 mm x 241 mm 3.0°C/s +/- 0,20 °C at 55 °C after 15 s no no Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0.1°C 260 mm x 440 mm x 241 mm 30 - 110°C Sealing foils, sealing mats, lid stripes, tube lids PCR tubes, high- and low-profile microplates (unskirted, semi-skirted, full-skirted), 8 well strips 1 - 240s/cycle +/- 0.1 °C to 20 °C per cycle

For more information about our products and possible variants, please refer to the technical documents or use our contact form.

Order Information

Order number

Description

846-2-070-301 Biometra TOne 96 G, 230 V, with 96 well block (0,2 ml) and gradient function
846-2-070-311 Biometra TOne 96, 230 V, with 96 well block (0,2 ml)

"The Biometra devices have proven to be very robust and reliable for complex PCR reactions."Prof. Dr. Ralf Küppers, Professor for Molecular Genetics at Institute for Cell Biology (Cancer Research), University of Duisburg-Essen

More customer success stories

Innovation and Experience for the Best PCR Results

Essential for research – gradient function

For rapid success, it is essential to have suitable tools at hand. For polymerase chain reactions (PCR), optimization of the annealing temperatures is crucial when it comes to achieving accurate results. In the Biometra TOne and Biometra TAdvanced systems, a temperature gradient over the long side of the block is realized as linearly as possible through special block construction and precise electronics. Users only need to enter the desired temperature step size and the annealing temperature for the middle of the block.

  • Optimization of the PCR protocol for new primer pairs in one run
  • Optimal annealing temperatures lead to the best specificity and yield
  • Easy transfer of results thanks to largely linear temperature steps from column to column
  • Quality through design, innovation and experiences

Biometra thermal cyclers are designed so users can rely on their results. The data accuracy of these systems pairs exceedingly well with its easy to use design.

  • A sophisticated temperature control system without exceeding or falling below the target temperature ensures transparency and maximum experimental reproducibility in all block positions.
  • The High-Performance Smart Lid (HPSL) ensures uniform temperatures and no condensation in the cover/block space, regardless of the plastic materials used.
  • The extremely quiet operation allows for the use of a large number of cyclers in labs with office areas.
  • A broad range of PCR plastics can be used due to the universal block design and adjusting cover pressure.
  • Everybody finds the right thermocycler for themselves from the range of block materials and formats.
  • The instruments come with low space requirements thanks to an efficient air flow design.

Accessories

Ethernet cable CAT 6 846-070-926

Software

PCR-Control-App

Easy and uncomplicated

In addition to simple programming directly on the device, Biometra thermal cyclers can be accessed through a network via smartphone or tablet. The PCR Control App also allows for the control and monitoring of devices outside of the laboratory, opening up completely new possibilities.The iOS- and Android-compatible software has much to offer:

  • Live monitoring of PCR runs
  • Starting and stopping runs
  • Saving and copying programs
  • Reading of parameters and notifications
Product Required Included
Biometra TOne 96 G, 230 V (846-2-070-301)
Biometra TOne 96, 230 V (846-2-070-311)

Biometra TSuite - Single License

Order number: 846-070-001

Multiple thermal cyclers, one place: Thanks to the Biometra TSuite software you can intuitively manage, program, and monitor multiple thermal cyclers from the comfort of your desk - regardless of how many you have or where they are located.

The Biometra TSuite thermal cycler management software gives you various possibilities to control and monitor your networked thermal cyclers from a computer. In the dashboard display, you can keep track of all important, current events and access active thermal cyclers directly. Through other windows, you can access detailed information on individual devices, programs, documentation files and general configuration options.

Benefit from many advantages in your everyday laboratory operations:

  • Suitable for all current models: Biometra TOne, Biometra TAdvanced, Biometra TRIO, Biometra TRobot II
  • Unlimited number of devices: Connect as many of your Biometra thermal cyclers as you want
  • Flexible usage: Choose between a mobile single license or multiple network licenses.
  • Compatible with existing devices: After a simple firmware update, you can use its full functionality.
Product Required Included
Biometra TOne 96 G, 230 V (846-2-070-301)
Biometra TOne 96, 230 V (846-2-070-311)

Consumables

844-70038-S 96 Well PCR Plate (0.2 ml; LP), full-skirted, white - 100 pcs
844-70087-12D Optical 8 Well Lid Chain, transparent, flat - 12 pcs
844-70087-0 Optical 8 Well Lid Chain, transparent, flat - 120 pcs
844-70043-5D Sealing foil (77 x 140 mm), adhesive, transparent, peelable - 5 pcs
844-70043-0 Sealing foil (77 x 140 mm), adhesive, transparent, peelable - 100 pcs
844-70044-5D Aluminum sealing foil (77 x 140 mm), adhesive, piercing and peelable - 5 pcs
844-70044-0 Aluminum sealing foil (77 x 140 mm), adhesive, piercing and peelable - 100 pcs
844-70036-0 96 Well PCR Plate (0.2 mL; HP), non-skirted, white - 100 pcs
844-70036-10D 96 Well PCR Plate (0.2 mL; HP), non-skirted, white - 10 pcs
844-70033-5D 96 Well PCR Plate (0.2 mL; HP), non-skirted, transparent - 5 pcs
844-70033-0 96 Well PCR Plate (0.2 mL; HP), non-skirted, transparent - 100 pcs
844-70037-5D 96 Well PCR Plate (0.2 mL; HP), half-skirted, white - 5 pcs
844-70037-0 96 Well PCR Plate (0.2 mL; HP), half-skirted, white - 100 pcs
844-70034-5D 96 Well PCR Plate (0.2 mL; HP), half-skirted, transparent - 5 pcs
844-70034-0 96 Well PCR Plate (0.2 mL; HP), half-skirted, transparent - 100 pcs
844-70035-10D 96 Well PCR Plate (0.2 mL; LP), full-skirted, transparent - 10 pcs
844-70035-0 96 Well PCR Plate (0.2 mL; LP), full-skirted, transparent - 100 pcs
844-70038-10S 96 Well PCR Plate (0.2 mL; LP), full-skirted, white - 10 pcs

Related Products

Biometra TAdvanced Series

The flexible high-quality thermal cycler with block quick-change system and 12 different block modules

Downloads

Br NGS Library Preparation (EN)
Brochure Biometra TMS Temperature Measuring System (EN)
Brochure PCR Biometra Thermal Cycler Series (EN)
Brochure Product Guide Life Science (EN)
Broschüre Biometra Thermocycler-Serie (DE)
Broschüre Biometra TMS Temperaturmesssystem (DE)
Broschüre Produktführer Life Science (DE)
Flyer Biometra TSuite Software (DE)
Flyer Biometra TSuite Software (EN)
Operating Manual Biometra TOne (DE)
Operating Manual Biometra TOne (EN)
Operating Manual Biometra TOne (ZH)
Product Guide Consumables for PCR/qPCR (EN)
Technical Data Biometra TOne (DE)
Technical Data Biometra TOne (EN)

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Biometra TOne – PCR Thermal Cycler (2024)

FAQs

What is the difference between a thermal cycler and a PCR? ›

Thermal Cycler (PCR Machine) The Thermal Cycler (also known as a Thermocycler, PCR Machine or DNA Amplifier) is a laboratory apparatus used to amplify segments of DNA via the Polymerase Chain Reaction (PCR). The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted.

What does a thermal cycler PCR machine do? ›

A PCR machine—or a thermal cycler—is an instrument that amplifies target nucleic acid sequences into millions of copies via polymerase chain reaction. Thermal cyclers inherit their name because they regulate temperatures in a cyclical program.

Can you do PCR without a thermal cycler? ›

And, if you refuse to spend any money on a thermocycler, you can make one from a lightbulb and a fan. Moreover, if you have access to a 3D printer, you can make your own qPCR (real-time PCR) machine.

What does thermal cycling do in PCR? ›

The actual PCR process is conducted via thermal cycling, a process of heating and cooling that creates the conditions necessary for DNA replication. Thermal cycling for PCR involves three phases-- denaturing, annealing, and extending. Denaturing: The first step in the thermal cycling process for PCR is denaturing.

How much DNA do you need to start with to do PCR? ›

The recommended starting DNA sample for the Somatic Mutation PCR Arrays is 500 ng for a 96-well plate, and 200 ng for a 384-well plate.

When would you use a thermocycler? ›

In molecular biology they are used for DNA sequencing, cloning, generation of probes, quantification of DNA and RNA, studying patterns of gene expression, detection of sequence-tagged sites, and many more techniques.

What are the three types of PCR? ›

Types of PCR
  • Multiplex PCR. Multiplex PCR employs different primer pairs in the same reaction for simultaneous amplification of multiple targets. ...
  • Long-range PCR. ...
  • Single-cell PCR. ...
  • Fast-cycling PCR. ...
  • Methylation-specific PCR (MSP) ...
  • Digital PCR. ...
  • Hot start PCR. ...
  • High-fidelity PCR.

How much is a PCR machine? ›

PCR machines will vary in price, ranging from around $3,000 to above $15,000 with higher-end models. Browse the catalog of thermocyclers below across leading suppliers and submit a quote for more information, including cost, specifications, and more.

Why does the lid of the thermocycler need to be heated? ›

Similarly, a heated lid is a common feature of thermal cyclers to prevent evaporation and condensation of PCR samples during runs. Prior to the introduction of the heated lid, samples were overlaid with mineral oil to achieve the same purpose.

What are 3 requirements needed before PCR can work? ›

The key ingredients of a PCR reaction are Taq polymerase, primers, template DNA, and nucleotides (DNA building blocks).

Can you run PCR without primers? ›

Without these components, DNA cannot be effectively amplified. Without primers PCR amplification is not possible.

Why might a PCR not work? ›

If the primer concentration is too low, annealing may be inefficient. Use well-designed primers at 0.2–1 μM in the final reaction. In addition, verify that the correct concentration was supplied by the manufacturer. If the polymerase concentration is too low, not all PCR products will be fully replicated.

What are the three steps to PCR thermocycling? ›

Three steps of PCR─denaturation, annealing, and extension─as shown in the first cycle, and the exponential amplification of target DNA with repeated cycling.

Are thermal cycler and PCR the same? ›

A PCR machine—or a thermal cycler—is an instrument that amplifies target nucleic acid sequences into millions of copies via polymerase chain reaction. Thermal cyclers inherit their name because they regulate temperatures in a cyclical program.

What is the purpose of thermal cycling? ›

Therefore, thermal cycling simulations are used to assess reliability and make appropriate material, geometry, and process modifications [63]. A thermal cycle is defined in terms of high and low temperatures, dwell times at high and low temperatures, and ramp rates between high and low temperatures. Fig.

How does PCR and a thermal cycler amplify your DNA sample? ›

The temperature cycles from 95°C to 50 to 60°C. The cycle is then repeated about 35 to 40 times using the thermal cycler which automatically repeats the heating and cooling cycles of the process. Resulting DNA sequence is doubled each time the heating/cooling cycle is conducted by the cycler.

What is the difference between PCR and isothermal PCR? ›

Temperature changes are the main difference between isothermal and PCR amplification methods. In PCR, a thermal cycler changes the reaction temperatures repeatedly to affect the actions of the temperature-dependent reagents; in contrast, an isothermal amplification reaction occurs at a single temperature.

How a thermal cycler helps with the process of PCR? ›

The thermocycler raises and lowers the temperature of the samples secured inside a holding block in discrete, pre-programmed steps, enabling double-strand denaturation, annealing, and polymerization.

What is the role of the thermal cycler in PCR quizlet? ›

95 degrees- thermocycler is used to increase the temperature of environment to 95 degrees to denature the template DNA strand. We now have two single strands of the original double helix, as the hydrogen bonds between the nitrogen bases are broken.

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