Biometra TAdvanced – PCR Thermal Cycler (2024)

Biometra TAdvanced 384 G, 230 V
846-2-070-214

Aluminum, special alloy 384 well microplate 5 - 25µL 4.0°C/s 3.8°C/s 2.0°C/s 1.7°C/s +/- 0,15 °C at 55 °C after 15 s yes 24 °C/0,1°C 24 rows 20 °C to 99°C yes Peltier elements Down to 4 °C Control Mode 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, sealing mats 384 PCR plates 1 - 240s/cycle +/-0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 384, 230 V
846-2-070-224

Aluminum, special alloy 384 well microplate 5 - 25µL 4.0°C/s 3.8°C/s 2.0°C/s 1.7°C/s +/- 0,15 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control Mode 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, sealing mats 384 well PCR plates 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 60 G, 230 V
846-2-070-200

Aluminum, special alloy 60 x 0,5 mL 20 - 150µL 6.0°C/s 5.5°C/s 4.0°C/s 3.5°C/s +/- 0,20 °C at 55 °C after 15 s yes 30 °C/0,1°C 10 rows 20 °C to 99°C yes Peltier elements Down to 4 °C Control Mode 3 - 99°C +/+ 0,1°C 30 - 110°C Tube lids PCR tubes 1- 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 60, 230 V
846-2-070-210

Aluminum, special alloy 60 x 0,5 mL tubes 20 - 150µL 6.0°C/s 5.5°C/s 4.0°C/s 3.5°C/s +/- 0,20 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Tube lids PCR tubes 1 - 240s/cycle +/- 0,1 °C to 20 C per cycle 10 cm at the rear side

Biometra TAdvanced 96 G, 230 V
846-2-070-201

Aluminum, special alloy 96 x 0,2 mL tubes, 96 well microplates or 8 well strips 5 - 50µL 6.0°C/s 5.5°C/s 4.0°C/s 3.5°C/s +/- 0,20 °C at 55 °C after 15 s yes 30 °C/0,1°C 12 rows 20 °C to 99°C yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, sealing mats, lid stripes, tube lids PCR tubes, high- and low profile microplates (unskirted, semi-skirted, full-skirted), 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 96 S, 230 V
846-2-070-251

Silver block, goald-coated 96 x 0,2 mL tubes, 96 well microplates or 8 well strips 5 - 100µL 8.0°C/s 7.0°C/s 5.5°C/s 4.5°C/s +/- 0,15 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Control Mode 3 - 99°C +/- 0,1°C 30 - 100°C Sealing foils, sealing mats, lid strips, tube lids PCR tubes, high- and low-profile microplates (unskirted, semi-skirted, full-skirted), 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 96 SG, 230 V
846-2-070-241

Silver block with gold coating 96 x 0,2 mL tubes, 96 well microplates or 8 well strips 5 - 100µL 8.0°C/s 7.0°C/s 5.5°C/s 4.5°C/s +/- 0,15 °C at 55 °C after 15 s yes 40 °C/0,1°C 12 rows 4 °C to 99°C yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, sealing mats, lid strips, tube lids PCR plates, high- and low-profile microplates (unskirted, semi-skirted, full-skirted), 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced 96, 230 V
846-2-070-211

Aluminum, special alloy 96 x 0.2 mL tubes, 96 well microplates or 8 well strips 5 - 50µL 6.0°C/s 5.5°C/s 4.0°C/s 3.5°C/s +/- 0,20 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, sealing mats, lid stripes, tube lids PCR tubes, high- and low-profile microplates (unskirted, semi-skirted, full-skirted), 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced Twin 30, 230 V
846-2-070-213

Aluminum, special alloy 2 x 30 x 0,5 mL tubes 20 - 200µL 4.2°C/s 4.1°C/s 3.3°C/s 3.1°C/s +/- 0,20 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C PCR tubes 0,5 mL 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced Twin 48 G, 230 V
846-2-070-202

Aluminum, special alloy 2 x 48 x 0,2 mL tubes, 2 x 48 well microplates, 2 x 6 x 8 well strips, 0,2 mL 5 - 70µL 5.2°C/s 5.1°C/s 4.1°C/s 4.0°C/s +/- 0,20 °C at 55 °C after 15 s yes 20 °C/0,1°C 8 rows 20 °C to 99°C yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, tube lids PCR tubes, 48 well microplates, 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced Twin 48, 230 V
846-2-070-212

Aluminum, special alloy 2 x 48 x 0,2 mL tubes, 2 x 48 well microplates, 2 x 6 x 8 well strips, 0,2 mL 5 - 70µL 5.2°C/s 5.1°C/s 4.1°C/s 4.0°C/s +/- 0,20 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, tube lids PCR tubes, 48 well microplates, 8 well strips 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side

Biometra TAdvanced Twin Combi, 230 V
846-2-070-215

Aluminum, special alloy 2 x 48 x 0,2 ml tubes, 2 x 48 well microplates, 2 x 6 x 8 well strips 0,2 ml, 2 x 18 (30 for tubes with small caps) x 0,5 ml 5 - 140µL 3.1°C/s 3.0°C/s 2.3°C/s 2.2°C/s +/- 0,20 °C at 55 °C after 15 s no yes Peltier elements Down to 4 °C Block Control 3 - 99°C +/- 0,1°C 30 - 110°C Sealing foils, lid stripes, tube lids PCR tubes (0,2 ml, 0,5 ml), 48 well microplates, 8 well strips (0,2 ml) 1 - 240s/cycle +/- 0,1 °C to 20 °C per cycle 10 cm at the rear side
Biometra TAdvanced – PCR Thermal Cycler (2024)

FAQs

What is the difference between a thermal cycler and a PCR? ›

Thermal Cycler (PCR Machine) The Thermal Cycler (also known as a Thermocycler, PCR Machine or DNA Amplifier) is a laboratory apparatus used to amplify segments of DNA via the Polymerase Chain Reaction (PCR). The device has a thermal block with holes where tubes holding the PCR reaction mixtures can be inserted.

What does a thermal cycler PCR machine do? ›

A PCR machine—or a thermal cycler—is an instrument that amplifies target nucleic acid sequences into millions of copies via polymerase chain reaction. Thermal cyclers inherit their name because they regulate temperatures in a cyclical program.

Can you do PCR without a thermal cycler? ›

And, if you refuse to spend any money on a thermocycler, you can make one from a lightbulb and a fan. Moreover, if you have access to a 3D printer, you can make your own qPCR (real-time PCR) machine.

How does a thermal cycler help with the process of PCR? ›

PCR machine steps

The solution contained in the tube is heated to at least 94°C (201.2°F) using a thermal cycler. The heat breaks the hydrogen bonds of the original DNA sample and separates the DNA into single strands (this is termed denaturation of double-stranded DNA).

When would you use a thermocycler? ›

In molecular biology they are used for DNA sequencing, cloning, generation of probes, quantification of DNA and RNA, studying patterns of gene expression, detection of sequence-tagged sites, and many more techniques.

How much DNA do you need to start with to do PCR? ›

The recommended starting DNA sample for the Somatic Mutation PCR Arrays is 500 ng for a 96-well plate, and 200 ng for a 384-well plate.

What are the three types of PCR? ›

Types of PCR
  • Multiplex PCR. Multiplex PCR employs different primer pairs in the same reaction for simultaneous amplification of multiple targets. ...
  • Long-range PCR. ...
  • Single-cell PCR. ...
  • Fast-cycling PCR. ...
  • Methylation-specific PCR (MSP) ...
  • Digital PCR. ...
  • Hot start PCR. ...
  • High-fidelity PCR.

How much is a PCR machine? ›

PCR machines will vary in price, ranging from around $3,000 to above $15,000 with higher-end models. Browse the catalog of thermocyclers below across leading suppliers and submit a quote for more information, including cost, specifications, and more.

Why is thermal cycling important in PCR? ›

The actual PCR process is conducted via thermal cycling, a process of heating and cooling that creates the conditions necessary for DNA replication. Thermal cycling for PCR involves three phases-- denaturing, annealing, and extending. Denaturing: The first step in the thermal cycling process for PCR is denaturing.

What are 3 requirements needed before PCR can work? ›

The key ingredients of a PCR reaction are Taq polymerase, primers, template DNA, and nucleotides (DNA building blocks).

Can you run PCR without primers? ›

Without these components, DNA cannot be effectively amplified. Without primers PCR amplification is not possible.

Why might a PCR not work? ›

If the primer concentration is too low, annealing may be inefficient. Use well-designed primers at 0.2–1 μM in the final reaction. In addition, verify that the correct concentration was supplied by the manufacturer. If the polymerase concentration is too low, not all PCR products will be fully replicated.

What is the difference between thermal cycler and PCR machine? ›

The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).

What is the purpose of PCR cycling? ›

PCR (Polymerase Chain Reaction) amplifies DNA and involves a series of temperature cycles critical for amplifying the DNA target. The cycling process is divided into three main stages: denaturation, annealing, and extension. These three stages are repeated for 20-40 cycles, doubling the targeted DNA amount.

Why is PCR important? ›

PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project. The technique is currently widely used by clinicians and researchers to diagnose diseases, clone and sequence genes, and carry out sophisticated quantitative and genomic studies in a rapid and very sensitive manner.

What does thermal cycling do in PCR? ›

The actual PCR process is conducted via thermal cycling, a process of heating and cooling that creates the conditions necessary for DNA replication. Thermal cycling for PCR involves three phases-- denaturing, annealing, and extending. Denaturing: The first step in the thermal cycling process for PCR is denaturing.

What is the difference between PCR and isothermal PCR? ›

Temperature changes are the main difference between isothermal and PCR amplification methods. In PCR, a thermal cycler changes the reaction temperatures repeatedly to affect the actions of the temperature-dependent reagents; in contrast, an isothermal amplification reaction occurs at a single temperature.

What is the role of the thermal cycler in PCR quizlet? ›

95 degrees- thermocycler is used to increase the temperature of environment to 95 degrees to denature the template DNA strand. We now have two single strands of the original double helix, as the hydrogen bonds between the nitrogen bases are broken.

What is the role of the thermal cycler in PCR multiple choice question? ›

The thermocycler raises and lowers the temperature of the samples secured inside a holding block in discrete, pre-programmed steps, enabling double-strand denaturation, annealing, and polymerization.

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